Microbes in Human Welfare - Competency Based & Assertion-Reasoning Questions
Practice questions testing deep understanding of biocontrol, biofertilizers, and industrial microbes.
Biotechnology and its Applications - Competency Based & Assertion-Reasoning Questions
Practice questions testing deep understanding of GM crops, insulin production, and RNA interference.
Created by Titas Mallick
Biology Teacher • M.Sc. Botany • B.Ed. • CTET (CBSE) • CISCE Examiner
Practice questions testing deep understanding of restriction enzymes, plasmids, and PCR.
Q1. A genetic engineer wants to cut a piece of human DNA and insert it into a bacterial plasmid. They cut the human DNA with the restriction enzyme EcoRI. A student suggests cutting the bacterial plasmid with a different restriction enzyme, BamHI, to save money. Explain biologically why the student's suggestion will completely ruin the experiment.
Q2. In the Polymerase Chain Reaction (PCR), the process involves heating the DNA to 94°C to separate the strands. Why must scientists use Taq polymerase in this machine instead of normal human DNA polymerase?
Q3. Assertion (A): A plasmid used as a cloning vector must contain a Selectable Marker, such as an antibiotic resistance gene (e.g., ampicillin resistance). Reason (R): The selectable marker allows scientists to easily distinguish and separate the bacteria that successfully took up the recombinant plasmid from the bacteria that failed to take it up (by growing them on an antibiotic plate).
/Class-12/Competency-Based-Questions/Unit_4_Chapter_1_Biotechnology_Principles_and_Processes_CBQ.mdxMicrobes in Human Welfare - Competency Based & Assertion-Reasoning Questions
Practice questions testing deep understanding of biocontrol, biofertilizers, and industrial microbes.
Biotechnology and its Applications - Competency Based & Assertion-Reasoning Questions
Practice questions testing deep understanding of GM crops, insulin production, and RNA interference.